Effect Natural Products Isolated From Vitex Agnus-Castus On Periostin In Mice Induced With Behcet Disease

Abstract

Behçet's disease is a rare and intriguing vasculitic condition marked by the persistent presence of oral and genital ulcers, along with a spectrum of systemic symptoms, including uveitis. Periostin is a carboxylate matrix protein that founded in kidney lung and bone, among other extracellular contain a hydrophilic COOH terminal domain, internal homologous repeat region cysteine-rich domains , and a signal peptide sequence at the NH2 end make up periostin. The extracellular matrix protein periostin, also known as osteoblast-specific factor-2,(OSF-2). In the present study Periostin, MDA,  TNF-alpha, IL-8, and GSH were measured by enzyme linked immune absorbent assay (ELISA). Biochemical parameters, creatinine that were measured by spectrophotometer technique in patients and healthy volunteers’ groups. The study included the separation and diagnosis of various natural products from the Vitex agnus-castus, where the oily substance and its fatty acids were separated, which were identified HPLC) technology. Some flavonoids and alkaloids were also separated. The flavonoids were identified by applying high-performance liquid chromatography (HPLC) technology. The effect of these products on Periostin was studied and it was found that they had a positive effect in reducing the level of Periostin and some other measured variables. In addition, the study examined the effect of these natural products on some biochemical parameters related to Behcet disease in the blood serum of mice induced with the disease. Biochemical variables included: Malondialdehyde (MDA), Glutathione (GSH), Creatinine, Interlukin-8 (IL-8), and Tumor necrosis alpha (TNF-alpha). The results showed that the concentrations of Periostin, MDA, IL-8 , TNF-alpha, and creatinine increased significantly in infected mice, compared to healthy mice, and a significant decrease in the concentration of GSH was observed.