Background: Diabetic neuropathy has an increasingly challenging clinician due to limited available therapeutic remedies, including vitamins, minerals, herbal remedies, and physical therapy. To evaluate the effect of vitamin B complex on diabetic peripheral neuropathy (DPNP) by assessment of nerve conductive study. Methods: Fifty diabetic patients with signs and symptoms of diabetic symmetrical peripheral neuropathy (DSPNP) enrolled in the study. A nerve conductive study (NCS) was done for all patients, according to the result of the NCS the patients were divided into 2 groups, group one includes 36 patients with mild to moderate DSPNP, and group two consisted of 14 patients with severe DSPNP, both groups received vitamins B for 3 months. NCS was repeated every month of treatment for 3 successive months. Results: In a mild and moderate group there is a significant improvement in nerve conductive studies (p<0.001), while in severe group patients there was no such improvement. Conclusion: The present study demonstrated that vitamin B complex has a beneficial effect on mild to moderate cases of patients with DPNP.